Research Associate, Sequencing (Mississauga)

KisoJi Biotechnology is a therapeutic antibody company that is developing mono- and multispecific antibody therapeutics by leveraging our state-of-the-art proprietary transgenic mouse (KisoMouse) platform for the generation of heavy chain-only antibodies (HCAbs). We are currently expanding our molecular biology team and are specifically looking for a Research Associate to expand our in-house Nanopore sequencing capabilities. This is an individual contributor position; the work requires on‑site laboratory bench work at our upcoming Toronto site expansion.Position: Research Associate Site: Toronto, Ontario, Canada Department: Molecular Biology Responsibilities

Establish, troubleshoot, and execute experimental pipelines for Nanopore sample preparation, quality‑control and sequencing Generate amplicon libraries for other NGS analyses Design, create and clone antibody/recombinant protein DNA constructs, and purify DNA (mini/midi/maxiprep) for subsequent transfection and testingAnalyze data and clearly communicate results in written reports and oral presentations Support the creation of SOPs as required Actively foster an setting of effective and collaborative working relationships within and across teams, and provide technical expertise in molecular biology methods Participate in team meetings to assist in the coordination of project and departmental activitiesEssential qualifications

M.Sc. in a relevant field (such as genetics, cell biology, or molecular biology) with at least 1-3 years of experience in a results-oriented environment (such as industry or a core facility) Significant experience with Nanopore MinION sequencing, from sample preparation and quality‑control to downstream results analysisExperience with molecular biology techniques such as cloning and DNA extraction (mini/midi/maxiprep) Independent technical troubleshooting skills Ability to accurately follow Standard Operating Procedures (SOPs) and instructions, and to perform repetitive tasks Strong time management skills, including the ability to effectively multi-task and work in a collaborative, fast-paced environmentStrong verbal and written communication skills, including the ability to document all work clearly and completely; proficient with Microsoft Office suite An enthusiasm for technology implementation rather than independent hypothesis-driven research Assets

Experience with other next-generation sequencing technologies (such as PacBio, Illumina) and library preparation is a plus Experience with the design and troubleshooting of cloning projects is a definite plus Experience in antibody discovery (e.g. phage display or mammalian display screening) is a plusBenefits

Benefits Package Includes Health / Dental Insurance Plans Paid Vacation and Holidays Incentive Programs Include Training&Development Programs / Courses Annual Performance Reviews with yearly bonuses Salary: The range for this position is $65,000-95,000. The base pay offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training.

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